Preditox : genetic assays for safety use

Publications

Updated : 05/27/2018

Le test γH2AX/pH3 nous a permis de démontrer le potentiel génotoxique de nombreux composés, ce qui a fait l'objet de nombreuses publications et interventions dans des congrès nationaux et internationaux.

2018

Publications

  • Theumeur, M., Henneb, Y., Khoury, L., Snini, S., Tadrist, S., Canlet, C., Puel, O., Oswald, I. and Audebert, M. (2018) Genotoxicity of aflatoxins and their precursors in human cells. Tox Letters 287:100-107.

Conférences ou posters en congrès

  • "Screening of the genotoxic potential of 160 mycotoxins in human cell lines." Audebert M., Khoury L., and Zalko, D. (46nd Annual Meeting of the European Environmental Mutagen Society, March 2018, Potsdam, Germany.).

2017

Publications

  • Kopp B, Zalko D, Audebert M. (2017) Genotoxicity of 11 heavy metals detected as food contaminants in two human cell lines. Environ Mol Mutagen. doi: 10.1002/em.22157.
  • Beaumont M, Portune KJ, Steuer N, Lan A, Cerrudo V, Audebert M, Dumont F, Mancano G, Khodorova N, Andriamihaja M, Airinei G, Tomé D, Benamouzig R, Davila AM, Claus SP, Sanz Y, Blachier F. (2017) Quantity and source of dietary protein influence metabolite production by gut microbiota and rectal mucosa gene expression: a randomized, parallel, double-blind trial in overweight humans. Am J Clin Nutr. 106(4):1005-1019.
  • Seiwert, N., Neitzel, C., Stroh, S., Frisan, T., Audebert, M., Toulany, M., Kaina, B. and Fahrer, J. (2017) AKT2 suppresses pro-survival autophagy triggered by DNA double-strand breaks. Cell Death and Disease, 8, e3019.
  • Liamin M, Boutet-Robinet E, Jamin EL, Fernier M, Khoury L, Kopp B, Le Ferrec E, Vignard J, Audebert M, Sparfel L. (2017) Benzo[a]pyrene-induced DNA damage associated with mutagenesis in primary human activated T lymphocytes. Biochem Pharmacol. 137:113-124.
  • Chevereau M, Glatt H, Zalko D, Cravedi JP, Audebert M. (2017) Role of human sulfotransferase 1A1 and N-acetyltransferase 2 in the metabolic activation of 16 heterocyclic amines and related heterocyclics to genotoxicants in recombinant V79 cells. Arch Toxicol. 91(9):3175-3184.

Conférences ou posters en congrès

  • "Génotoxicité des multi-expositions à des produits agro-alimentaires - Aspects pratiques" Audebert M. (Société Française de Toxicologie 2017, November 2017, Paris, France).
  • "Screening of the genotoxic potential of 160 mycotoxins in human cell lines. " Audebert M., Khoury L., and Zalko, D. (12 th International Conference on Environmental Mutagens ICEM 2017, November 2017, Seoul, Korea).
  • "Role of phase I and II metabolic enzymes in the metabolic activation of heterocyclic amines genotoxins. " Audebert M., Chevereau M., Glatt H., Cravedi JP and Zalko, D. (48 nd Annual Meeting of the Environmental Mutagenesis and Genomics Society, September 2017, Raleigh, USA.)
  • "In vitro Genotoxicity and Mutagenicity of Food Contaminant Mixtures present in the Diet." Kopp. B., Le Hégarat, L., Zalko, D. and Audebert M. (48 nd Annual Meeting of the Environmental Mutagenesis and Genomics Society, September 2017, Raleigh, USA.)
  • "Complementarity of cellular biomarkers quantification for characterizing genotoxic mechanisms of action in the HepG2 cell line." Kopp. B., Dario, M., Zalko, D. and Audebert M. (45 nd Annual Meeting of the European Environmental Mutagen Society, June 2017, Bruxelles, Belgium.)
  • "Complementarity of cellular biomarkers quantification for characterizing genotoxic mechanisms of action in the HepG2 cell line." Kopp. B., Dario, M., Zalko, D. and Audebert M. (Annual Meeting of the Genetic Toxicology Association, May 2017, Philadelphia, USA.)

2016

Publications

  • Beaumont M., Andriamihaja M., Lan A., Khodorova N., Audebert M., Blouin JM., Grauso M., Lancha L., Benamouzig R., Tomé D., Bouillaud F., Davila AM. and Blachier F. (2016) Detrimental effects for colonocytes of an increased exposure to luminal hydrogen sulfide: The adaptive response. Free Radic Biol Med. 93:155-64.
  • Khoury, L., Zalko, D. and Audebert, M. (2016) Complementarity of phosphorylated histones H2AX and H3 quantification and different cell lines for genotoxic screening. Archives of Toxicology, 90(8):1983-95.
  • Khoury, L., Zalko, D. and Audebert, M. (2016) Evaluation of four human cell lines with distinct biotransformation properties for genotoxic screening. Mutagenesis 31(1):83-96.
  • Quesnots, N., Rondel, K., Martinais, S., Audebert, M., Glaise, D., Morel, F., Loyer, P. and Robin MA. (2016) Evaluation of genotoxicity using automated detection of gH2AX in metabolically competent HepaRG cells. Mutagenesis 31(1):43-50.

Conférences ou posters en congrès

  • « Development of a new in vitro high-throughput genotoxic screening strategy in human cells » Audebert M. (The 19th International Congress on In Vitro Toxicology, October 2016, Juan-les-Pins, France)
  • « Development of a new in vitro high-throughput genotoxic screening strategy in human cells » Audebert M. (Annual Meeting of the Environmental Mutagen Society (EMS), September 2016, Kansas city, USA)
  • « Complementarity of phosphorylated histones H2AX and H3 quantification and different cell lines for genotoxic screening » Audebert M. (44nd Annual Meeting of the European Environmental Mutagenesis and Genomics Society, August 2016, Prague, Czech Republic)
  • « Vers l’évaluation du risque à haut-débit » Audebert M. (SFTG 2016, June 2016, Paris, France)

2014 et 2015

Publications

  • Andriamihaja M., Lan A., Beaumont M., Audebert M., Wong X., Yamada K., Yin Y., Tomé D., Carrasco-Pozo C., Gotteland M., Kong X. and Blachier F. (2015) The deleterious metabolic and genotoxic effects of the bacterial metabolite p-cresol on colonic epithelial cells. Free Radic Biol Med.: 85:219-27.
  • Bastide NM, Chenni F, Audebert M, Santarelli RL, Taché S, Naud N, Baradat M, Jouanin I, Surya R, Hobbs DA, Kuhnle GG, Raymond-Letron I, Gueraud F, Corpet DE, Pierre FH. (2015) A central role for heme iron in colon carcinogenesis associated with red meat intake. Cancer Res.;75(5):870-9.
  • Conférences ou posters en congrès

    • « Le point sur les DNA damage kits » Audebert M. (SFTG 2015, July 2015, Caen, France)
    • « A high throughput genotoxicity screen using γH2AX » Audebert M. (43nd Annual Meeting of the European Environmental Mutagen Society, July 2014, Lancaster, UK)

    2013

    Publications

    • Khoury, L., Zalko, D. and Audebert, M. (2013) Validation of High-Throughput Genotoxicity Assay Screening Using the γH2AX In-Cell Western Assay on HepG2 Cells. Environ Mol Mutagen, 54, 737-46.
    • Martin O., Santarelli R., Taché S., Naud N., Guéraud F., Audebert M., Dupuy J., Meunier N., Attaix D., Vendeuvre J.L., Mirvish S., Kuhnle G., Cano N. and Corpet D. (2013) Calcium and α-Tocopherol Suppress Cured Meat Promotion of Chemically-Induced Colon Carcinogenesis in Rats and Reduce Associated Biomarkers in Human Volunteers. American Journal of Clinical Nutrition, 98, 1255-62.
    • Martin P., Marcq I., Magistro G., Penary M., Garcie C., Payros D., Boury M,. Olier M., Nougayrede J.P., Audebert M., Chalut C., Schubert S., and Oswald E. (2013) Interplay between Siderophores and Colibactin Genotoxin Biosynthetic Pathways in Escherichia coli. PLoS Pathogen 9(7):e1003437.
    • Crépet A., Héraud F., Béchaux C., Gouze M.E., Pierlot S., Fastier A., Leblanc J.C., Le Hégarat L., Takakura N., Fessard V;, Tressou J., Maximilien R., de Sousa G;, Nawaz A., Zucchini-Pascal N., Rahmani R., Audebert, M. Graillot, V., and Cravedi, J.P. (2013) The PERICLES research program: An integrated approach to characterize the combined effects of mixtures of pesticide residues to which the French population is exposed. Toxicology, 313, 83-93.
    • Jamin, E.L., Riu, A., Douki, T., Debrauwer, L., Cravedi, J.P., Zalko, D. and Audebert, M. (2013) Combined genotoxic effects of a polycyclic aromatic hydrocarbon (B(a)P) and an heterocyclic amine (PhIP) in relation to colorectal carcinogenesis. Plos ONE, 8(3):e58591.

    Conférences ou posters en congrès

    • « Validation of a high-throughput genotoxic screening using γH2AX in cell western assay on humans cells. » Audebert, M., Khoury L. (ICEM 2013, November 2013, Iguazu, Brazil)
    • « Validation of a high-throughput genotoxic screening using γH2AX in cell western assay on humans cells. » Audebert, M., Khoury L. (SFTG 2013, October 2013, Strasbourg, France)

    2012

    Publications

    • Graillot, V., Tomasetig, F., Cravedi, J.P. and Audebert, M. (2012) Evidence of the in vitro genotoxicity of methyl-pyrazole pesticides in human cells. Mutat Res., 748(1-2):8-16.
    • Graillot, V., Takakura, N., Hegarat, L., Fessard, V., Audebert, M. and Cravedi, J.P. (2012) Genotoxicity of pesticide mixtures present in the diet of the French population. Environ Mol Mutagen, 53, 173-84.
    • Audebert, M., Zeman, F., Beaudoin, R., Pery, A. and Cravedi, J.P. (2012) Comparative potency approach based on H2AX assay for estimating the genotoxicity of polycyclic aromatic hydrocarbons. Toxicol Appl Pharmacol, 260, 58-64.

    Conférences ou posters en congrès

    • « Food mutagens in relation to risk of colorectal carcinogenesis.» Audebert, M. (Oxidative DNA Damage: From Chemical Aspects to Biological Consequences, January 2012, Les Houches, France)

    2009 à 2011

    Publications

    • Audebert, M., Dolo, L., Perdu, E., Cravedi, J. P., and Zalko, D. (2011). Use of the γH2AX assay for assessing the genotoxicity of bisphenol A and bisphenol F in human cell lines. Archives of Toxicology 85(11):1463-73.
    • Riu, A., Le Maire, A., Grimaldi, M., Audebert, M., Hillenweck, A., Bourguet, W., Balaguer, P., and Zalko, D. (2011). Characterization of novel ligands of ERα, ERβ and PPARγ: the case of halogenated bisphenol A and their conjugated metabolites Toxicological Sciences 122 372-82.
    • M. Audebert, A. Riu, C. Jacques, E. L. Hillenweck, E. Jamin, D. Zalko, and J. P. Cravedi (2010). Use of the γH2AX assay for assessing the role of metabolism in the genotoxicity of polycyclic aromatic hydrocarbons in human cell lines. Toxicology Letters, 199, 182–192

    Conférences ou posters en congrès

    • « Metabolism and Genotoxicity of Food Contaminants: Description of a New Genotoxic Assay. » M. Audebert (41st Annual Meeting of the Environmental Mutagen Society (EMS), October 2010, Fort Worth, USA)
    • « Gamma-H2AX : avantages et limites dans la détection de la génotoxicité. » M. Audebert (SFTG 2010, May 2010, Villejuif, France)
    • « Appréciation quantitative de l'exposition alimentaire à des mélanges de pesticides et mécanismes de génotoxicité.» Graillot, V., Audebert, M. (Congress of the French group of pesticides, May 2009, Toulouse, France)
    • « Effect of Polycyclic Aromatic Hydrocarbons (PAHs) xenobiotics metabolism on DNA integrity, Development of a new genotoxic assay.» Audebert, M. (Oxidative DNA Damage: From Chemical Aspects to Biological Consequences, January 2009, Les Houches, France)