Les tests γH2AX et pH3 nous ont permis de démontrer le potentiel génotoxique de nombreux composés, ce qui a fait l'objet de nombreuses publications et interventions dans des congrès nationaux et internationaux.

 

2019

Publications

  • Comparative UHPLC-HRMS Profiling, Toxicological Assessment, and Protection Against H2O2-Induced Genotoxicity of Different Parts of Opuntia ficus indica.

    Tsafantakis N, Katsanou ES, Kyriakopoulou K, Psarou EC, Raptaki I, Skaltsounis AL, Audebert M, Machera KA and Fokialakis N. J Med Food. 2019 Dec;22(12):1280-1293. doi: 10.1089/jmf.2019.0032. Epub 2019 Oct 9.

  • Validation of the γH2AX biomarker for genotoxicity screening: a review.

    Kopp B, Khoury L. and Audebert M. Arch. Toxicol. 2019 Aug;93(8):2103-2114. doi: 10.1007/s00204-019-02511-9. Epub 2019 Jul 9.

  • Determination of genotoxic potencies of pyrrolizidine alkaloids in HepaRG cells using the γH2AX assay.

    Louisse J, Rijkers D, Stoopen G, Holleboom WJ, Delagrange M, Molthof E, Mulder PPJ, Hoogenboom RLAP, Audebert M, Peijnenburg AACM. Food Chem Toxicol. 2019 Sep;131:110532. doi: 10.1016/j.fct.2019.05.040. Epub 2019 May 30.

Congrès

  • "Use of the gH2AX/pH3 assay to investigate MOA of food contaminants."

    Audebert, M. Oral. EEMGS. Rennes - France, May 2019

2018

Congrès

  • “Genotoxic and carcinogenic potential of 160 mycotoxins in human cells”

    Audebert M. Oral. Francopa, Paris – France, November 2018

  • Screening of the genotoxic potential of 160 mycotoxins in human cell lines""

    Audebert M. Poster. ESTIV, Berlin – Germany, October 2018

  • Lésions et réparation de l’ADN: utilité de la technique CRISPR-Cas9 pour l’étude de la génotoxicité.""

    Audebert M. Oral. SFTG/SFCM, Paris – France, October 2018

  • Screening of the genotoxic potential of 160 mycotoxins in human cell lines." "

    Audebert M., Khoury L., and Zalko, D. Oral. 46nd Annual Meeting of the European Environmental Mutagen Society, Potsdam - Germany, March 2018

  • “Genotoxic and carcinogenic potential of 160 mycotoxins in human cells”

    Audebert M. Poster. SFT, Lille - France, November 2018

Publications

  • An Untargeted Metabolomics Approach to Investigate the Metabolic Modulations of HepG2 Cells Exposed to Low Doses of Bisphenol A and 17β-Estradiol.

    Cabaton NJ, Poupin N, Canlet C, Tremblay-Franco M, Audebert M, Cravedi JP, Riu A, Jourdan F, Zalko D. Front Endocrinol (Lausanne). 2018 Sep 25;9:571. doi: 10.3389/fendo.2018.00571. 

  • High-protein diets for weight management: Interactions with the intestinal microbiota and consequences for gut health. A position paper by the my new gut study group.

    Blachier F, Beaumont M, Portune KJ, Steuer N, Lan A, Audebert M, Khodorova N, Andriamihaja M, Airinei G, Benamouzig R, Davila AM, Armand L, Rampelli S, Brigidi P, Tomé D, Claus SP, Sanz Y. Clin Nutr. 2018 Sep 20. pii: S0261-5614(18)32454-3. doi:10.1016/j.clnu.2018.09.016. 

  • Genotoxicity and mutagenicity assessment of food contaminant mixtures present in the French diet.

    Kopp B, Vignard J, Mirey G, Fessard V, Zalko D, Le Hgarat L, Audebert M. Environ Mol Mutagen. 2018 Oct;59(8):742-754. doi: 10.1002/em.22214.

  • Assessment of a panel of cellular biomarkers and the kinetics of their induction in comparing genotoxic modes of action in HepG2 cells.

    Kopp B, Dario M, Zalko D, Audebert M. Environ Mol Mutagen. 2018 Jul;59(6):516-528. doi: 10.1002/em.22197.

  • Genotoxicity of aflatoxins and their precursors in human cells.

    Theumer MG, Henneb Y, Khoury L, Snini SP, Tadrist S, Canlet C, Puel O, Oswald IP, Audebert M. Toxicol Lett. 2018 May 1;287:100-107. doi: 10.1016/j.toxlet.2018.02.007.

  • Genotoxicity of 11 heavy metals detected as food contaminants in two human cell lines.

    Kopp B, Zalko D, Audebert M. Environ Mol Mutagen. 2018 Apr;59(3):202-210. doi: 10.1002/em.22157.

2017

Congrès

  • In vitro Genotoxicity and Mutagenicity of Food Contaminant Mixtures present in the Diet.""

    Kopp. B., Le Hégarat, L., Zalko, D. and Audebert M. (48 nd Annual Meeting of the Environmental Mutagenesis and Genomics Society, September 2017, Raleigh, USA.)

  • Complementarity of cellular biomarkers quantification for characterizing genotoxic mechanisms of action in the HepG2 cell line.""

    Kopp. B., Dario, M., Zalko, D. and Audebert M. (45 nd Annual Meeting of the European Environmental Mutagen Society, June 2017, Bruxelles, Belgium.)

  • Complementarity of cellular biomarkers quantification for characterizing genotoxic mechanisms of action in the HepG2 cell line.""

    Kopp. B., Dario, M., Zalko, D. and Audebert M. (Annual Meeting of the Genetic Toxicology Association, May 2017, Philadelphia, USA.)

  • Génotoxicité des multi-expositions à des produits agro-alimentaires - Aspects pratiques" "

    Audebert M. (Société Française de Toxicologie 2017, November 2017, Paris, France).

  • Screening of the genotoxic potential of 160 mycotoxins in human cell lines. ""

    Audebert M., Khoury L., and Zalko, D. (12 th International Conference on Environmental Mutagens ICEM 2017, November 2017, Seoul, Korea).

  • Role of phase I and II metabolic enzymes in the metabolic activation of heterocyclic amines genotoxins. " "

    Audebert M., Chevereau M., Glatt H., Cravedi JP and Zalko, D. (48 nd Annual Meeting of the Environmental Mutagenesis and Genomics Society, September 2017, Raleigh, USA.)

Publications

  • Quantity and source of dietary protein influence metabolite production by gut microbiota and rectal mucosa gene expression: a randomized, parallel, double-blind trial in overweight humans.

    Beaumont M, Portune KJ, Steuer N, Lan A, Cerrudo V, Audebert M, Dumont F, Mancano G, Khodorova N, Andriamihaja M, Airinei G, Tomé D, Benamouzig R, Davila AM, Claus SP, Sanz Y, Blachier F. Am J Clin Nutr. 2017 Oct;106(4):1005-1019. doi: 10.3945/ajcn.117.158816.

  • AKT2 suppresses pro-survival autophagy triggered by DNA double-strand breaks in colorectal cancer cells.

    Seiwert N, Neitzel C, Stroh S, Frisan T, Audebert M, Toulany M, Kaina B, Fahrer J. Cell Death Dis. 2017 Aug 24;8(8):e3019. doi: 10.1038/cddis.2017.418.

  • Benzo[a]pyrene-induced DNA damage associated with mutagenesis in primary human activated T lymphocytes.

    Liamin M, Boutet-Robinet E, Jamin EL, Fernier M, Khoury L, Kopp B, Le Ferrec E, Vignard J, Audebert M, Sparfel L. Biochem Pharmacol. 2017 Aug 1;137:113-124. doi: 10.1016/j.bcp.2017.04.025.

  • Role of human sulfotransferase 1A1 and N-acetyltransferase 2 in the metabolic activation of 16 heterocyclic amines and related heterocyclics to genotoxicants in recombinant V79 cells.

    Chevereau M, Glatt H, Zalko D, Cravedi JP, Audebert M. Arch Toxicol. 2017 Sep;91(9):3175-3184. doi: 10.1007/s00204-017-1935-8.

2016

Congrès

  • « Vers l’évaluation du risque à haut-débit » 

    Audebert M. (SFTG 2016, June 2016, Paris, France)

  • « Development of a new in vitro high-throughput genotoxic screening strategy in human cells » 

    Audebert M. (The 19th International Congress on In Vitro Toxicology, October 2016, Juan-les-Pins, France)

  • « Development of a new in vitro high-throughput genotoxic screening strategy in human cells » 

    Audebert M. (Annual Meeting of the Environmental Mutagen Society (EMS), September 2016, Kansas city, USA)

  • « Complementarity of phosphorylated histones H2AX and H3 quantification and different cell lines for genotoxic screening » 

    Audebert M. (44nd Annual Meeting of the European Environmental Mutagenesis and Genomics Society, August 2016, Prague, Czech Republic)

Publications

  • Detrimental effects for colonocytes of an increased exposure to luminal hydrogen sulfide: The adaptive response.

    Beaumont M, Andriamihaja M, Lan A, Khodorova N, Audebert M, Blouin JM, Grauso M, Lancha L, Benetti PH, Benamouzig R, Tomé D, Bouillaud F, Davila AM, Blachier F. Free Radic Biol Med. 2016 Apr;93:155-64. doi: 10.1016/j.freeradbiomed.2016.01.028

  • Complementarity of phosphorylated histones H2AX and H3 quantification in different cell lines for genotoxicity screening.

    Khoury L, Zalko D, Audebert M. Arch Toxicol. 2016 Aug;90(8):1983-95. doi: 10.1007/s00204-015-1599-1.

  • Evaluation of genotoxicity using automated detection of γH2AX in metabolically competent HepaRG cells.

    Quesnot N, Rondel K, Audebert M, Martinais S, Glaise D, Morel F, Loyer P, Robin MA. Mutagenesis. 2016 Jan;31(1):43-50. doi: 10.1093/mutage/gev059.

  • Evaluation of four human cell lines with distinct biotransformation properties for genotoxic screening.

    Khoury L, Zalko D, Audebert M. Mutagenesis. 2016 Jan;31(1):83-96. doi: 10.1093/mutage/gev058. 

2015

Publications

  • The deleterious metabolic and genotoxic effects of the bacterial metabolite p-cresol on colonic epithelial cells.

    Andriamihaja M, Lan A, Beaumont M, Audebert M, Wong X, Yamada K, Yin Y, Tomé D, Carrasco-Pozo C, Gotteland M, Kong X, Blachier F. Free Radic Biol Med. 2015 Aug;85:219-27. doi: 10.1016/j.freeradbiomed.2015.04.004.

  • A central role for heme iron in colon carcinogenesis associated with red meat intake.

    Bastide NM, Chenni F, Audebert M, Santarelli RL, Taché S, Naud N, Baradat M, Jouanin I, Surya R, Hobbs DA, Kuhnle GG, Raymond-Letron I, Gueraud F, Corpet DE, Pierre FH. Cancer Res. 2015 Mar 1;75(5):870-9. doi: 10.1158/0008-5472.CAN-14-2554

Congrès

  • « Le point sur les DNA damage kits » 

    Audebert M. (SFTG 2015, July 2015, Caen, France)

  • « A high throughput genotoxicity screen using γH2AX » 

    Audebert M. (43nd Annual Meeting of the European Environmental Mutagen Society, July 2014, Lancaster, UK)

2013

Congrès

  • « Validation of a high-throughput genotoxic screening using γH2AX in cell western assay on humans cells. » 

    Audebert, M., Khoury L. (SFTG 2013, October 2013, Strasbourg, France)

  • « Validation of a high-throughput genotoxic screening using γH2AX in cell western assay on humans cells. »

    Audebert, M., Khoury L. (ICEM 2013, November 2013, Iguazu, Brazil)

Publications

  • Validation of high-throughput genotoxicity assay screening using γH2AX in-cell western assay on HepG2 cells.

    Khoury L, Zalko D, Audebert M. Environ Mol Mutagen. 2013 Dec;54(9):737-46. doi: 10.1002/em.21817.

  • Calcium and α-tocopherol suppress cured-meat promotion of chemically induced colon carcinogenesis in rats and reduce associated biomarkers in human volunteers.

    Pierre FH, Martin OC, Santarelli RL, Taché S, Naud N, Guéraud F, Audebert M, Dupuy J, Meunier N, Attaix D, Vendeuvre JL, Mirvish SS, Kuhnle GC, Cano N, Corpet DE. Am J Clin Nutr. 2013 Nov;98(5):1255-62. doi: 10.3945/ajcn.113.061069.

  • Interplay between siderophores and colibactin genotoxin biosynthetic pathways in Escherichia coli.

    Martin P, Marcq I, Magistro G, Penary M, Garcie C, Payros D, Boury M, Olier M, Nougayrède JP, Audebert M, Chalut C, Schubert S, Oswald E. PLoS Pathog. 2013;9(7):e1003437. doi: 10.1371/journal.ppat.1003437.

  • The PERICLES research program: an integrated approach to characterize the combined effects of mixtures of pesticide residues to which the French population is exposed.

    Crépet A, Héraud F, Béchaux C, Gouze ME, Pierlot S, Fastier A, Leblanc JCh, Le Hégarat L, Takakura N, Fessard V, Tressou J, Maximilien R, de Sousa G, Nawaz A, Zucchini-Pascal N, Rahmani R, Audebert M, Graillot V, Cravedi JP. Toxicology. 2013 Nov 16;313(2-3):83-93. doi: 10.1016/j.tox.2013.04.005.

  • Combined genotoxic effects of a polycyclic aromatic hydrocarbon (B(a)P) and an heterocyclic amine (PhIP) in relation to colorectal carcinogenesis.

    Jamin EL, Riu A, Douki T, Debrauwer L, Cravedi JP, Zalko D, Audebert M. PLoS One. 2013;8(3):e58591. doi: 10.1371/journal.pone.0058591.

2012

Publications

  • Evidence of the in vitro genotoxicity of methyl-pyrazole pesticides in human cells.

    Graillot V, Tomasetig F, Cravedi JP, Audebert M. Mutat Res. 2012 Oct 9;748(1-2):8-16. doi: 10.1016/j.mrgentox.2012.05.014.

  • Genotoxicity of pesticide mixtures present in the diet of the French population.

    Graillot V, Takakura N, Hegarat LL, Fessard V, Audebert M, Cravedi JP. Environ Mol Mutagen. 2012 Apr;53(3):173-84. doi: 10.1002/em.21676.

  • Comparative potency approach based on H2AX assay for estimating the genotoxicity of polycyclic aromatic hydrocarbons.

    Audebert M, Zeman F, Beaudoin R, Péry A, Cravedi JP. Toxicol Appl Pharmacol. 2012 Apr 1;260(1):58-64. doi: 10.1016/j.taap.2012.01.022.

Congrès

  • « Food mutagens in relation to risk of colorectal carcinogenesis.» 

    Audebert, M. (Oxidative DNA Damage: From Chemical Aspects to Biological Consequences, January 2012, Les Houches, France)

2011

Congrès

  • « Effect of Polycyclic Aromatic Hydrocarbons (PAHs) xenobiotics metabolism on DNA integrity, Development of a new genotoxic assay.» 

    Audebert, M. (Oxidative DNA Damage: From Chemical Aspects to Biological Consequences, January 2009, Les Houches, France)

  • « Metabolism and Genotoxicity of Food Contaminants: Description of a New Genotoxic Assay. » 

    M. Audebert (41st Annual Meeting of the Environmental Mutagen Society (EMS), October 2010, Fort Worth, USA)

  • « Gamma-H2AX : avantages et limites dans la détection de la génotoxicité. » 

    M. Audebert (SFTG 2010, May 2010, Villejuif, France)

  • « Appréciation quantitative de l'exposition alimentaire à des mélanges de pesticides et mécanismes de génotoxicité.»

    Graillot, V., Audebert, M. (Congress of the French group of pesticides, May 2009, Toulouse, France)

Publications

  • Use of the γH2AX assay for assessing the genotoxicity of bisphenol A and bisphenol F in human cell lines.

    Audebert M, Dolo L, Perdu E, Cravedi JP, Zalko D. Arch Toxicol. 2011 Nov;85(11):1463-73. doi: 10.1007/s00204-011-0721-2.

  • Characterization of novel ligands of ERα, Erβ, and PPARγ: the case of halogenated bisphenol A and their conjugated metabolites.

    Riu A, le Maire A, Grimaldi M, Audebert M, Hillenweck A, Bourguet W, Balaguer P, Zalko D. Toxicol Sci. 2011 Aug;122(2):372-82. doi: 10.1093/toxsci/kfr132.

  • Use of the γH2AX assay for assessing the genotoxicity of polycyclic aromatic hydrocarbons in human cell lines.

    Audebert M, Riu A, Jacques C, Hillenweck A, Jamin EL, Zalko D, Cravedi JP. Toxicol Lett. 2010 Nov 30;199(2):182-92. doi: 10.1016/j.toxlet.2010.08.022.